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Biotin --- Affinity labeling --- Immunoadsorption --- Affinity chromatography

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This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports. Key Features * Matrix activation * Ligand immobilization * Binding and elution of target molecules * Enzyme catalysis on solid supports * Analytical affinity chromatography * Isolation/purification of antibodies * Preparation of antibody fragments * Immunoaffinity chromatography * Immobilization of nucleic acids * Use of immobilized ligands for removal of trace contaminants Practical advice on choosing: * Matrices * Spacers * Methods of activation and coupling Background information and insights on: * Affinity interactions * The ease and power of affinity chromatography * Attaching molecules to insoluble supports * Matrices currently in use * Over 20 methods of activation * Spacers * Extensive References

Chromatography, Affinity. --- Ligands. --- Protein Binding. --- Affinity chromatography --- Immobilized ligands (Biochemistry) --- Immunoadsorption --- Ligand binding (Biochemistry) --- Binding, Ligand (Biochemistry) --- Biochemistry --- Dye-ligand affinity chromatography --- Radioligand assay --- Adsorption (Biology) --- Antigen-antibody reactions --- Immunochemistry --- Ligands (Biochemistry) --- Chromatographic analysis --- Affinity Chromatography --- Bioaffinity Chromatography --- Chromatography, Bioaffinity --- Affinity chromatography. --- Immunoadsorption. --- Basic Sciences. Biology --- Biological Techniques --- Immobilized ligands (Biochemistry). --- Ligand binding (Biochemistry). --- Biological Techniques. --- Chromatography, Affinity --- Ligands --- Protein Binding --- Immunochromatography --- BIOCHEMISTRY --- immunoadsorption --- ligand binding --- immobilized ligands --- Monograph --- Biochemistry. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Ligand --- Plasma Protein Binding Capacity --- Binding, Protein --- Binding Sites --- Blood Proteins --- Protein Interaction Mapping

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Blood --- Hemapheresis --- Leukapheresis --- Sang --- Leucophérèse --- Plasmaphérèse --- Hemapheresis. --- Leukapheresis. --- Plasmapheresis --- Transfusion --- Périodiques. --- Transfusion. --- Plasmaphereses --- Leukocytopheresis --- Lymphocytopheresis --- Lymphopheresis --- Leukocytapheresis --- Leukopheresis --- Lymphapheresis --- Lymphocytapheresis --- Leukaphereses --- Leukocytaphereses --- Leukocytophereses --- Leukophereses --- Lymphaphereses --- Lymphocytaphereses --- Lymphocytophereses --- Lymphophereses --- Leucapheresis --- Leucopheresis --- Apheresis of blood --- Blood component removal --- Blood component separation --- Pheresis of blood --- Removal of blood components --- Separation of blood components --- Blood transfusion therapy --- Hemotherapy --- Transfusion medicine --- Transfusion of blood --- Transfusion therapy --- Transfusion therapy, Blood --- Apheresis --- Pheresis --- Blood Transfusion --- Blood Transfusions --- Transfusion, Blood --- Transfusions, Blood --- Cell Separation --- Cell separation --- Leucocytes --- Surgery --- Blood banks --- Blood groups --- Hospitals --- Transfusion-free surgery --- Body fluids --- Fear of blood --- Collection and preservation --- Transfusion committees --- Blood Transfusion. --- Plasmapheresis. --- Double Filtration Plasmapheresis --- Therapeutic Immunoadsorption --- Therapeutic Plasma Adsorption --- Therapeutic Plasmapheresis --- Adsorption, Therapeutic Plasma --- Adsorptions, Therapeutic Plasma --- Double Filtration Plasmaphereses --- Filtration Plasmapheresis, Double --- Immunoadsorption, Therapeutic --- Plasma Adsorption, Therapeutic --- Plasmapheresis, Double Filtration --- Plasmapheresis, Therapeutic --- Therapeutic Immunoadsorptions --- Therapeutic Plasma Adsorptions --- Therapeutic Plasmaphereses --- Hémaphérèse --- Leucophérèse. --- Plasmaphérèse. --- Periodicals. --- Plasma exchange (Therapeutics) --- Blood Donation

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Apheresis refers to an extracorporeal therapy which aims at removing pathological constituents from the patients’ blood. Due to the development of new techniques as well as the discovery of novel autoimmune antibodies, it is increasingly recognized as an important therapeutic option for a variety of autoimmune-mediated neurological disorders, including multiple sclerosis, myasthenia gravis, autoimmune encephalitis, Guillain–Barré syndrome, and many others. Therapeutic plasma exchange (TPE) constitutes the standard method of apheresis for most indications, while immunoadsorption (IA) offers a more specific, low-risk alternative. Both methods aim at removing auto-antibodies from the blood. Evidence for most neurological diseases is still low. Interestingly, more recent developments suggest that apheresis is not limited to the removal of autoantibodies but may also be useful in neurodegenerative and possibly even in acute vascular disorders.

Medicine --- immunoadsorption --- acute relapsing multiple sclerosis --- plasma exchange --- therapeutic apheresis --- multiple sclerosis --- optic neuritis --- relapse --- class IV --- steroids --- Alzheimer’s clinical syndrome --- dementia --- autoantibodies --- α1-Adrenergic receptor --- Inflammatory neuropathy --- chronic inflammatory demyelinating polyneuropathy --- Guillain-Barré syndrome --- paranodal antibodies --- plasmapheresis --- Myalgic Encephalomyelitis/Chronic Fatigue Syndrome --- ß2 adrenoreceptor autoantibody --- autoimmune encephalitis --- limbic encephalitis --- NMDAR (N-Methyl-D-Aspartat) --- antibody --- paraneoplastic --- apheresis --- therapeutic plasma exchange --- neurological diseases --- CRP --- stroke --- inflammation --- n/a --- Alzheimer's clinical syndrome --- Guillain-Barré syndrome

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Hemapheresis --- Blood --- Cell separation --- Leukapheresis --- Plasmapheresis --- Sang --- Cellules --- Leucophérèse --- Plasmaphérèse --- Cell separation. --- Hemapheresis. --- Leukapheresis. --- Plasmapheresis. --- Blood Transfusion, Autologous. --- Transfusion --- Transfusion, Autologous --- Autotransfusion --- Séparation --- Transfusion. --- Transfusion, Autologous. --- Titles of journals. --- Titles of electronic journals. --- Autologous Blood Transfusion --- Autologous Blood Transfusions --- Blood Transfusions, Autologous --- Transfusion, Autologous Blood --- Transfusions, Autologous Blood --- Autotransfusions --- Leucapheresis --- Leucopheresis --- Leukopheresis --- Apheresis of blood --- Blood component removal --- Blood component separation --- Pheresis of blood --- Removal of blood components --- Separation of blood components --- Cell isolation --- Cell segregation --- Cells --- Blood transfusion therapy --- Hemotherapy --- Transfusion medicine --- Transfusion of blood --- Transfusion therapy --- Transfusion therapy, Blood --- Apheresis --- Pheresis --- Separation --- Autologous blood transfusion --- Autoreinfusion of blood --- Autotransfusion of blood --- Blood Transfusion, Autologous --- Cell Separation --- Leukocytopheresis --- Lymphocytopheresis --- Lymphopheresis --- Leukocytapheresis --- Lymphapheresis --- Lymphocytapheresis --- Leukaphereses --- Leukocytaphereses --- Leukocytophereses --- Leukophereses --- Lymphaphereses --- Lymphocytaphereses --- Lymphocytophereses --- Lymphophereses --- Isolation, Cell --- Cell Isolation --- Cell Segregation --- Cell Isolations --- Cell Segregations --- Cell Separations --- Isolations, Cell --- Segregation, Cell --- Segregations, Cell --- Separation, Cell --- Separations, Cell --- Plasmaphereses --- Operative Blood Salvage --- Plasma exchange (Therapeutics) --- Leucocytes --- Cytometry --- Separation (Technology) --- Surgery --- Blood banks --- Blood groups --- Hospitals --- Transfusion-free surgery --- Body fluids --- Fear of blood --- Collection and preservation --- Transfusion committees --- Cell Separation. --- Double Filtration Plasmapheresis --- Therapeutic Immunoadsorption --- Therapeutic Plasma Adsorption --- Therapeutic Plasmapheresis --- Adsorption, Therapeutic Plasma --- Adsorptions, Therapeutic Plasma --- Double Filtration Plasmaphereses --- Filtration Plasmapheresis, Double --- Immunoadsorption, Therapeutic --- Plasma Adsorption, Therapeutic --- Plasmapheresis, Double Filtration --- Plasmapheresis, Therapeutic --- Therapeutic Immunoadsorptions --- Therapeutic Plasma Adsorptions --- Therapeutic Plasmaphereses --- Hémaphérèse --- Leucophérèse. --- Plasmaphérèse. --- Autotransfusion. --- Séparation. --- Blood Donation